ASTM D5392
Standard Test Method for Isolation and Enumeration of Escherichia coli in Water by the Two-Step Membrane Filter Procedure
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About This Item
ASTM D5392 is a laboratory standard focused on isolating and counting Escherichia coli in water using a two-step membrane filter procedure. It provides a structured approach for detecting this indicator organism in water samples, helping support consistent test results and clearer comparison between laboratories. By defining a standard method for enumeration, ASTM D5392 supports water quality testing, routine monitoring, and technical decision-making where reliable microbiological data is important.
ASTM D5392 standard overview
The official title of ASTM D5392 identifies a Standard Test Method for isolation and enumeration of Escherichia coli in water by a two-step membrane filter procedure. In practical terms, this means the standard is intended to guide a controlled analytical workflow for capturing, isolating, and counting the target organism from water samples. As an ASTM standard, it serves as a common technical reference for laboratories that need a repeatable method and a consistent basis for reporting results.
Where is ASTM D5392 used?
ASTM D5392 is typically used in water testing settings where Escherichia coli measurements are needed for monitoring or verification purposes. It may be applied by laboratories handling drinking water, environmental water, or other water sample types where microbiological assessment is part of the workflow. The method is also relevant for organizations that rely on membrane filtration equipment and standardized incubation or counting procedures to evaluate water quality with greater consistency.
Practical importance of ASTM D5392
The practical value of ASTM D5392 is its ability to support consistent and defensible microbiological testing. A standardized method helps reduce variation in sample handling, isolation, and enumeration, which can improve comparability across testing programs. For procurement, compliance, and quality control activities, ASTM D5392 offers a recognized technical reference that can help support reliable reporting and reduce uncertainty when assessing the presence and count of Escherichia coli in water.
- Water sample isolation and counting
- Two-step membrane filter procedure
- Escherichia coli enumeration
- Laboratory consistency and repeatability
- Quality control and reporting support
- Publication Date: 2024-04-10
- Publisher: ASTM
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